Fachdokumente Online der Landesanstalt für Umwelt Baden-Württemberg

zur LUBW   zum Ministerium für Umwelt, Klima und Energiewirtschaft   zum Ministerium für Ländlichen Raum und Verbraucherschutz   zum Ministerium für Verkehr und Infrastruktur  

Short term exposure to inert dust alters cytology and cytokine release in human nasal mucosa

Summary

Recent epidemiological investigations have confirmed the impact of particulate matter on human respiratory health. The pathophysiological alterations induced in human respiratory mucosa following exposure to various aerosols are incompletely understood.

In this project we investigated the effects of toxicologically inert dust on human nasal mucosa in a human short term exposure setting. Thirtytwo healthy volunteers were exposed to calcium-carbonate dust (mean mass aerodynamic 15 µm) in concentrations of 150, 500 and 1000 µg/m 3 for 3 hours. Exposure to filtered air served as control. Directly and 8 hours following exposure, nasal cytologies and nasal secretions were obtained. Cytologies were stained with anti- CD45, myeloperoxidase, -CD68 and -tryptase. In the epithelial lining fluid IL-1.... , IL-6, IL-8, TNF-a , MCP-1, MIP-1..., ENA-78 and substance P were measured using an ELISA technique. The results suggests that toxicologically inert particulate matter may cause a faint proinflammatory reaction of human nasal mucosa likely due to mechanical and sensible irritation. However this effect is weak and can not explain the profound effects of environmental particulate matter on human respiratory health. It is assumed that the toxicity of various compounds of environmental particulate matter are primarily relevant. The results of this study may provide reliable information to distinguish the effects of toxic components in environmental particulate matter from mechanical and sensible irritation.

 


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